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A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
- Publication Year :
- 2021
- Publisher :
- Research Square Platform LLC, 2021.
-
Abstract
- Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address diverse imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micron), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols of different refractive indices. We present a hybrid system that combines a novel non-orthogonal dual-objective and conventional open-top light-sheet architecture for highly versatile multi-scale volumetric imaging. One sentence summary Glaser et al. describe a hybrid open-top light-sheet microscope to image cleared tissues at mesoscopic to sub-micron resolution and depths of up to 1 cm.
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........e7f98796627fd56a18f1fec8472f08c1