Diversity and intratumoral heterogeneity in human gallbladder cancer progression revealed by single-cell RNA sequencing

Gallbladder cancer (GC) is a malignant disease characterized with highly cellular heterogeneity and poor prognosis . Determine the intratumoral heterogeneity and tumor microenvironment (TME) can provide novel therapeutic strategies for GC. We performed the single-cell RNA sequencing (scRNA-seq) analysis on the cells dissociated from the primary, lymph node metastatic and the adjacent normal tissues of 5 GC patients. The transcriptomic atlas and the ligand-receptor based intercellular communication network between the cells were elucidated.