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Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium
- Source :
- Methods in Molecular Biology ISBN: 9781493997312
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- Arthropod brains are fascinating structures that exhibit great complexity but also contain conserved elements that can be recognized between species. There is a long tradition of research in insect neuroanatomy, cell biology, and in studying the genetics of insect brain development. Recently, the beetle Tribolium castaneum has gained attention as a model for insect head and brain development, and many anterior patterning genes have so far been characterized in beetle embryos. The outcome of embryonic anterior development is the larval and, subsequently, the adult brain. A basic requirement to understand genetic cell type diversity within these structures is the ability to localize mRNA and protein of neural genes. Here we detail our protocols for RNA in situ hybridization in combination with immunohistochemistry, optimized for dissected brains of larval and adult beetles.
- Subjects :
- 0303 health sciences
Messenger RNA
Cell type
media_common.quotation_subject
fungi
In situ hybridization
Insect
Biology
biology.organism_classification
3. Good health
Cell biology
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
Gene expression
medicine
Red flour beetle
Gene
030217 neurology & neurosurgery
030304 developmental biology
media_common
Neuroanatomy
Subjects
Details
- ISBN :
- 978-1-4939-9731-2
- ISBNs :
- 9781493997312
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493997312
- Accession number :
- edsair.doi...........e886039cc8b8586c31b8b7ba0a3e1300