Nucleic Acid Probes for Detection and Strain Discrimination of Cucurbit Geminiviruses

With a probe composed of four unique full-length DNA geminiviral genome components cloned from squash infected with two variants of squash leaf curl virus (SLCV), viral nucleic acid could be detected in various infected hosts representing nine genera from four plant families. Two biologically different strains could be differentiated with these fragment probes and mixtures of these two strains could be detected in experimentally infected plants