Detection of peroxynitrite anion released from HeLa cells via hyper-cross-linked polymer nanotube modified electrode

Abnormal expression levels of peroxynitrite anion (ONOO−) in human cells have relation to many diseases, especially cancer. Here, we constructed a novel sensing platform for directly detecting ONOO− using hyper-cross-linked polymeric nanotubes (HNTs)–modified glassy carbon electrode. The HNTs nanohybrids were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), FT-IR spectroscopy, and cyclic voltammetry (CV). The prepared HNTs/GCE sensor exhibited outstanding performance in detecting ONOO−, which provided a wide linear range from 2.34 × 10−8 M to 1.74 × 10−4 M and a low detection limit of 7.43 nM (S/N = 3). Moreover, it was also used to measure the ONOO− released from HeLa cells induced by stimulus. The designed sensor may provide great potential for expand applications in the matter of cancer treatment and biomedicine.