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Protein Crystallization Irradiated by Audible Sound: The Effect of Varying Sound Frequency
- Source :
- Crystal Growth & Design. 19:258-267
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Protein crystallization is a process that is very sensitive to the physical environment. Audible sound is an environmental characteristic that can significantly affect the crystallization process. Previously, it was found that the crystallization result is frequency dependent, that is, the crystallization of protein under different sound frequencies yields different results. Here, we further investigate the effect of varying frequency (or a frequency program) on protein crystallization. Twelve different frequency programs and six proteins were used to test the effect of varying sound frequency on protein crystallization. The results showed that varying the audible sound frequency from high to low exhibited the most significant improvement in protein crystallization. Varying frequency linearly from 15 000 to 100 Hz in 12 h best promoted crystallization, with the average number of crystallization hits 36.5% higher than in the control. Crystal quality was improved with sound irradiation using STW2 program. O...
- Subjects :
- geography
geography.geographical_feature_category
Materials science
010405 organic chemistry
Analytical chemistry
General Chemistry
010402 general chemistry
Condensed Matter Physics
01 natural sciences
0104 chemical sciences
law.invention
Crystal
Quality (physics)
law
General Materials Science
Irradiation
Crystallization
Protein crystallization
Sound (geography)
Audio frequency
Subjects
Details
- ISSN :
- 15287505 and 15287483
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Crystal Growth & Design
- Accession number :
- edsair.doi...........ea4ecce94f2423bc001fb99fefbf507e