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Single-Cell Analysis of Long Noncoding RNAs (lncRNAs) in Mouse Brain Cells
- Source :
- Methods in Molecular Biology ISBN: 9781071611579
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- Single-cell analysis has contributed greatly to gaining a better understanding of human brain function and has implications for neurodegenerative and neuropsychiatric disorders. Long noncoding RNAs (lncRNAs) acting, in part, as epigenetic regulators exist in brain cells in high abundance exhibiting a large diversity that play important roles in neural development, function, and neurodegenerative disease. Due to lncRNA tissue-type and cell-type specific expression characteristics, it is important to analyze lncRNA at single-cell resolution. In this chapter, we highlight a method named scTISA (single-cell transcription in situ with antisense RNA amplification), which is applicable to fixed single cells and can yield polyA+ lncRNAs and mRNAs data at the same time.
- Subjects :
- 0303 health sciences
Computational biology
Human brain
Biology
Antisense RNA
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
Single-cell analysis
Single cell sequencing
Transcription (biology)
medicine
Epigenetics
Neural development
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-07-161157-9
- ISBNs :
- 9781071611579
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071611579
- Accession number :
- edsair.doi...........eb3a5e83fb92cf77b737841877822476