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Etude comparative des activités antioxydantes d’extraits éthanoliques de feuilles, d’écorces et de racines de Cassia sieberiana

Authors :
Messanvi Gbeassor
Kodjo Selom Evenamede
Pakoupati Boyode
Kafui Kpegba
Oudjaniyobi Simalou
Amegnona Agbonon
Source :
International Journal of Biological and Chemical Sciences. 11:2924
Publication Year :
2018
Publisher :
African Journals Online (AJOL), 2018.

Abstract

Dans cet article, les 3 organes de C. sieberiana recoltee au Togo ont fait l’objet d’une etude comparative pour la determination de leur composition phytochimique, teneur en phenols et flavonoides totaux, et activites antioxydantes. Le tri phytochimique a ete realise grâce aux reactions de colorimetrie et precipitation. La teneur en phenols totaux a ete determinee grâce au reactif du Folin-Ciocalteu et celle des flavonoides par AlCl 3 . L’activite antioxydante a ete evaluee par les methodes de DPPH et FRAP. Les resultats obtenus ont montre que les 3 organes de la plante contiennent des grands groupes chimiques, tels que les flavonoides, tanins et saponosides. Les teneurs en phenols totaux des extraits ethanoliques sont (en mg EAG/g) de 276,62 ± 26,120 (feuilles) ; 345,04 ± 3,160 (ecorces) ; 327,16 ± 3,990 (racines). Celles des flavonoides totaux sont (en mg EQ/g) de 34,134 ± 4,324 (feuilles) ; 36,430 ± 2,022 (ecorces) et 37,270 ± 2,216 (racines). Les extraits ethanoliques des 3 organes ont un pouvoir antioxydant appreciable. En conclusion, les resultats obtenus revelent que les racines et ecorces ont des activites antioxydantes semblables, toutefois ne permettent pas a ce stade de la recherche de preciser l’organe approprie pouvant remplacer les racines. © 2017 International Formulae Group. All rights reserved. Mots cles: Cassia sieberiana ; composition phytochimique activite antioxydante ; phenols totaux English Title: Comparative antioxidant potential study of different part of Cassia sieberiana English Abstract In this article, the 3 organs of C. sieberiana harvested in Togo were the subject of a comparative study for the determination of their phytochemical composition, content of total phenols and flavonoids, and antioxidant activities. Phytochemical composition was carried out by colorimetric and precipitation reactions. The total phenol content was determined by Folin-Ciocalteu reagent and total flavonoids by AlCl 3 . Antioxidant activity was evaluated by DPPH and FRAP methods. The results obtained showed that the 3 organs of the plant contain large chemical groups, such as flavonoids, tannins and saponosides. The total phenol contents of the ethanolic extracts are (in mg EAG / g) of 276.62 ± 26.120 (leaves); 345.04 ± 3.160 (barks); 327.16 ± 3.990 (roots). Those of the total flavonoids are (in mg Eq / g) 34,134 ± 4,324 (leaves); 36,430 ± 2,022 (barks) and 37,270 ± 2,216 (roots). The ethanolic extracts of the 3 organs have an appreciable antioxidant power. In conclusion, the results obtained reveal that the roots and barks have similar antioxidant activities, however do not allow at this stage of research to specify the appropriate organ that can replace the roots. © 2017 International Formulae Group. All rights reserved. Keywords: Cassia sieberiana ; phytochemical composition; antioxidant activity; total phenols

Details

ISSN :
1997342X and 19918631
Volume :
11
Database :
OpenAIRE
Journal :
International Journal of Biological and Chemical Sciences
Accession number :
edsair.doi...........eb770bbdfac1b2f1c805baca665aa2c3
Full Text :
https://doi.org/10.4314/ijbcs.v11i6.29