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Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction

Authors :
Sarah E. Maddocks
Rowena Jenkins
Publication Year :
2017
Publisher :
Elsevier, 2017.

Abstract

This chapter provides practical advice on what needs to be addressed before undertaking polymerase chain reaction (PCR). From keeping the workspace nuclease free to recipes and shopping lists; information that is vital know and understand before putting on a lab coat and entering the lab, is described. The reader is guided through the practical aspects of preparing to undertake PCR with examples of reagents provided as well as a list of common manufacturers of PCR reagents. Safety considerations are discussed and thought is given to equipment and reagents that will be required for analysis of the first PCR reaction.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........ebd3b7f98f6296f3d8fe9a87936316d1
Full Text :
https://doi.org/10.1016/b978-0-12-802683-0.00001-0