Back to Search
Start Over
Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage
- Source :
- SPIE Proceedings.
- Publication Year :
- 2007
- Publisher :
- SPIE, 2007.
-
Abstract
- Microcantilevers (MCs) as state-of-art sensor platforms have been widely investigated. We recently introduced a new type of MC, magnetostrictive microcantilever (MSMC), as high performance sensor platform. The MSMC is a remote/wireless sensor platform and exhibits a high quality merit factor in liquid. In this paper, a MSMC-based biosensor is developed for detecting B. anthracis spores in liquid, a potential biothreaten agent. The results demonstrated the advantages of MSMCs as a sensor platform. MSMCs with different sizes were fabricated and utilized in the experiments. The MSMCs were coated with the filamentous phage as a bio-recognition element to capture the B. anthracis spores. The phage-coated MSMCs as biosensors were exposed to cultures containing target spores with concentrations ranging from 5 * 10 4 spores/mL to 5 * 10 8 spores/mL. The resonance frequency of the MSMC sensors in cultures was monitored in a real-time manner. The results showed that for MSMCs of 2.8 mm * 1.0 mm * 35 mm and with 1.4 mm * 0.8 mm * 35 mm have a detection limit of 10 5 and 10 4 spores/mL, respectively.
Details
- ISSN :
- 0277786X
- Database :
- OpenAIRE
- Journal :
- SPIE Proceedings
- Accession number :
- edsair.doi...........ec605b5f274cd21a3287580d032ddc92