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Heat transfer time determination based on DNA melting curve analysis
- Source :
- Microfluidics and Nanofluidics. 24
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The determination of the physical properties of fluids—such as the thermal characteristics, which include heat transfer time (Δt)—is becoming more challenging as system sizes shrink to micro- and nanometer scales. Hence, knowledge of these properties is crucial for the operation of devices requiring precise temperature (T) control, such as polymerase chain reactions, melting curve analysis (MCA), and differential scanning fluorimetry. In this paper, we introduced a flow-through microfluidic system to analyze thermal properties such as Δt among samples and the sidewall of a silicon chip using microscopic image analysis. We performed a spatial MCA with double-stranded deoxyribonucleic acid (dsDNA) and EvaGreen intercalator, using a flow-through microfluidic chip, and achieved a T gradient of ≈ 2.23 K mm−1. We calculated the mean value of Δt as ≈ 33.9 ms from a melting temperature (TM) location shift along the microchannel for a variable flow rate. Our system had a T resolution of ≈ 1.2 mK pixel−1 to distinguish different dsDNA molecules—based on the TM location within the chip—providing an option to use it as a high-throughput device for rapid DNA or protein analysis.
- Subjects :
- Microchannel
Materials science
010401 analytical chemistry
Microfluidics
Resolution (electron density)
Analytical chemistry
02 engineering and technology
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
Fluorescence spectroscopy
Melting curve analysis
0104 chemical sciences
Electronic, Optical and Magnetic Materials
Heat transfer
Thermal
Materials Chemistry
Nanometre
0210 nano-technology
Subjects
Details
- ISSN :
- 16134990 and 16134982
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Microfluidics and Nanofluidics
- Accession number :
- edsair.doi...........ed4b4a537060e6a3da7ec013d46eefa7