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Enzymatic degradation, antioxidant and immunoregulatory activities of polysaccharides from brown algae Sargassum fusiforme
- Source :
- Journal of Food Measurement and Characterization. 15:1960-1972
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- In order to make full use of brown algae Sargassum fusiforme, in the presence of pectinase and glucoamylase, the polysaccharide (SFP) from S. fusiforme was hydrolyzed to improve its biological activity. Single factor tests and response surface methodology were used to optimize enzymatic hydrolysis conditions based upon the degradation rate of SFP. Conditions for the hydrolysis of SFP were optimized as: hydrolysis temperature 54.2 °C, total enzyme concentration 68.5 U/mL, activity ratio of pectinase to glucoamylase 3.3:1, pH 6.0, degradation time 3 h. Under the optimized conditions, the degradation rate of SFP reached 18.6%. The antioxidant activities of the enzymatically degraded S. fusiforme polysaccharide (ESFP) prepared under the optimized conditions were greatly improved when compared to those of SFP. The IC50 values of ESFP on scavenging HO˙, $${\text{O}}_2^{\cdot -}$$ and DPPH˙ were 4.78, 0.412 and 0.53 mg/mL, respectively; while those for SFP were 6.82, 0.723 and 0.768 mg/mL, respectively. The ferric reducing power of ESFP was also significantly higher than that of SFP. After incubation with RAW264.7 macrophages for 24 and 48 h, ESFP was found to possess significantly stronger activities in promoting cell proliferation, phagocytosis and NO release than those of SFP, indicating the superior immunoregulatory activity of ESFP.
- Subjects :
- Antioxidant
General Chemical Engineering
medicine.medical_treatment
Polysaccharide
01 natural sciences
Industrial and Manufacturing Engineering
Hydrolysis
0404 agricultural biotechnology
Enzymatic hydrolysis
medicine
Food science
Pectinase
Safety, Risk, Reliability and Quality
chemistry.chemical_classification
biology
Chemistry
010401 analytical chemistry
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
0104 chemical sciences
Brown algae
Enzyme
Ferric
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 21934134 and 21934126
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Food Measurement and Characterization
- Accession number :
- edsair.doi...........ef770dc20e2ab76be47965eee73571eb