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Orientation assignment of LH2 and LH1-RC complexes from Thermochromatium tepidum reconstituted in PC liposome and their ultrafast excitation dynamics comparison between in artificial and in natural chromatophores
- Source :
- Chemical Physics Letters. 705:78-84
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- In this study, the artificial chromatophore was constructed by reconstitution of light-harvesting complex 2 (LH2) and core-complex (LH1-RC) from Thermochromatium tepidum in egg phosphatidylcholine (PC) liposome. By calcium ion dependent absorption changes, the orientations of protein complexes in liposomes solely were assigned. Ultrafast EET dynamics were studied by fs time-resolved absorption spectroscopy on both the artificial and natural chromatophores at their RC ‘open’ and ‘close’ states, respectively. The results indicated that these two membrane systems have similar EET dynamic profiles, and the orientations of LH2 and LH1-RC complexes in artificial chromatophores were opposite to those in natural chromatophores.
- Subjects :
- 0301 basic medicine
Liposome
030102 biochemistry & molecular biology
Absorption spectroscopy
Chemistry
Dynamics (mechanics)
General Physics and Astronomy
Chromatophore
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Membrane
Phosphatidylcholine
Biophysics
Physical and Theoretical Chemistry
Absorption (chemistry)
Excitation
Subjects
Details
- ISSN :
- 00092614
- Volume :
- 705
- Database :
- OpenAIRE
- Journal :
- Chemical Physics Letters
- Accession number :
- edsair.doi...........f02d9190dab88cfad9301ff0a668a757
- Full Text :
- https://doi.org/10.1016/j.cplett.2018.05.043