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Orientation assignment of LH2 and LH1-RC complexes from Thermochromatium tepidum reconstituted in PC liposome and their ultrafast excitation dynamics comparison between in artificial and in natural chromatophores

Authors :
Yang-Lin Jiang
Jian-Ping Zhang
Peng Wang
Li-Ming Tan
Ying Shi
Ling Dai
Zheng-Yu Otomo
Source :
Chemical Physics Letters. 705:78-84
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

In this study, the artificial chromatophore was constructed by reconstitution of light-harvesting complex 2 (LH2) and core-complex (LH1-RC) from Thermochromatium tepidum in egg phosphatidylcholine (PC) liposome. By calcium ion dependent absorption changes, the orientations of protein complexes in liposomes solely were assigned. Ultrafast EET dynamics were studied by fs time-resolved absorption spectroscopy on both the artificial and natural chromatophores at their RC ‘open’ and ‘close’ states, respectively. The results indicated that these two membrane systems have similar EET dynamic profiles, and the orientations of LH2 and LH1-RC complexes in artificial chromatophores were opposite to those in natural chromatophores.

Details

ISSN :
00092614
Volume :
705
Database :
OpenAIRE
Journal :
Chemical Physics Letters
Accession number :
edsair.doi...........f02d9190dab88cfad9301ff0a668a757
Full Text :
https://doi.org/10.1016/j.cplett.2018.05.043