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Cloning of a Resistance Gene Analog from Wheat and Development of a Codominant PCR Marker for Pm21
- Source :
- Journal of Integrative Plant Biology. 48:715-721
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- To investigate the mechanism of resistance to wheat (Triticum aestivum L.) powdery mildew, suppression subtractive hybridization was conducted between an isogenic resistant line carrying Pm21 and its recurrent parent Yangmai 5 to isolate the resistance relative genes. A cDNA fragment specifically expressed in the resistant line was obtained and its full length was cloned by in silico cloning and RT-PCR. This gene encoded a deduced protein of 219 amino acids with a leucine-rich repeat (LRR) motif, often found in plant resistance genes, and was designated as Ta-LRR2. Ta-LRR2 had an increased expression level in the resistant line after inoculation with Erysiphe graminis DC. f. sp. tritici Marchal. PCR analysis with different cytogenetic stocks suggested that Ta-LRR2 was specifically associated with chromosome arms 6VS and 6AS. Linkage analysis further showed that Ta-LRR2 could be used as a resistance gene analog polymorphism marker of Pm21 for marker-assisted selection in germplasm enhancement and breeding practice. Moreover, how to isolate Pm21 based on the information obtained for Ta-LRR2 is discussed. (Managing editor: Li-Hui Zhao)
- Subjects :
- Genetics
Cloning
Germplasm
food and beverages
Plant Science
Biology
Biochemistry
Molecular biology
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
chemistry
Genetic linkage
Suppression subtractive hybridization
Complementary DNA
Molecular marker
Gene
Powdery mildew
Subjects
Details
- ISSN :
- 17447909 and 16729072
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Plant Biology
- Accession number :
- edsair.doi...........f11a3ac397035e62628011a18304c66c