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Single Live-Bacterial Cell Assay of Promoter Activity and Regulation

Authors :
Ayako Kori
Akira Ishihama
E. Shohata
Toshio Fukuda
Fumihito Arai
Akiko Hasegawa
Yukio Magariyama
Yu-Ching Lin
E.-S.H. Magdy
Masahiro Nakajima
Yoko Yamanishi
Jun Teramoto
Source :
2008 International Symposium on Micro-NanoMechatronics and Human Science.
Publication Year :
2008
Publisher :
IEEE, 2008.

Abstract

For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture using the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of E. coli gcl promoter strength.

Details

Database :
OpenAIRE
Journal :
2008 International Symposium on Micro-NanoMechatronics and Human Science
Accession number :
edsair.doi...........f130809e75efbac6b4eb6095fbab9686
Full Text :
https://doi.org/10.1109/mhs.2008.4752467