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Template-assisted Cu2O@Fe(OH)3 yolk-shell nanocages as biomimetic peroxidase: A multi-colorimetry and ratiometric fluorescence separated-type immunosensor for the detection of ochratoxin A
- Source :
- Journal of Hazardous Materials. 411:125090
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The convenient and effective detection of toxins is urgently demanded for food security and human health. Herein, based on the catalytic activity of mimetic peroxidase from the Cu2O@Fe(OH)3 yolk-shell nanocages, a dual-modal multi-colorimetric and ratiometric fluorescence immunosensor for the sensitive detection of ochratoxin A (OTA) was successfully developed. For the multi-colorimetric detection, H2O2 can be effectively decomposed by Cu2O@Fe(OH)3 to form ·OH groups, thus Au nanorods (Au NRs) can be etched to exhibit vivid color variations and localized surface plasmon resonance (LSPR) shifts. For the ratiometric fluorescence detection, o-phenylenediamine was oxidized by Cu2O@Fe(OH)3 to form 2,3-diaminophenazine (DAP) in the presence of H2O2. Interestingly, the exogenous fluorescence signal source of carbon dots can be quenched by DAP via inner filter effect, while a new emission peak at 563 nm can be discovered, forming a ratiometric fluorescence signal. Due to the independent signals and mutual confirmation, the performance of the dual-modal immunosensor for the detection of OTA was significantly improved, where a broad linear range from 1 ng/L to 10 μg/L with a detection limit of 0.56 ng/L (S/N = 3) was achieved. The sensing strategy was also used to monitor OTA in millet and lake water samples with a satisfied performance.
- Subjects :
- Detection limit
021110 strategic, defence & security studies
Environmental Engineering
biology
Chemistry
Health, Toxicology and Mutagenesis
0211 other engineering and technologies
02 engineering and technology
010501 environmental sciences
01 natural sciences
Pollution
Fluorescence
Nanocages
Linear range
biology.protein
Environmental Chemistry
Nanorod
Surface plasmon resonance
Colorimetry
Waste Management and Disposal
0105 earth and related environmental sciences
Peroxidase
Nuclear chemistry
Subjects
Details
- ISSN :
- 03043894
- Volume :
- 411
- Database :
- OpenAIRE
- Journal :
- Journal of Hazardous Materials
- Accession number :
- edsair.doi...........f237e3e006506d977726f260bb8e9801