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Candelabrum cells are molecularly distinct, ubiquitous interneurons of the cerebellar cortex with specialized circuit properties

Authors :
Naeem Nadaf
Wade G. Regehr
Evan Z. Macosko
Stephanie Rudolph
Tomas Osorno
Wei-Chung Allen Lee
Tri Nguyen
Kozareva
Publication Year :
2021
Publisher :
Cold Spring Harbor Laboratory, 2021.

Abstract

To understand how the cerebellar cortex transforms mossy fiber (MF) inputs into Purkinje cell (PC) outputs, it is vital to delineate the elements of this circuit. Candelabrum cells (CCs) are enigmatic interneurons of the cerebellar cortex that have been identified based on their morphology, but their electrophysiological properties, synaptic connections, and function remain unknown. Here we clarify these properties using electrophysiology, snRNA sequencing, in situ hybridization, and serial electron microscopy. We find that CCs are the most abundant PC layer interneuron. They are GABAergic, molecularly distinct, and present in all cerebellar lobules. Their high resistance renders CC firing highly sensitive to synaptic inputs. CCs are excited by MFs and granule cells, and strongly inhibited by PCs. CCs in turn inhibit molecular layer interneurons, which leads to PC disinhibition. Thus, inputs, outputs and local signals all converge onto CCs to allow them to assume a unique role in controlling cerebellar output.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........f3b03b9a935fe1394fb545032d4b2f2e
Full Text :
https://doi.org/10.1101/2021.04.09.439172