Manzamine C, an alkaloid indole as an inhibitor of the cancer cells adapted to nutrient starvation, from an Indonesian marine sponge of Xestospongia muta

Research of Manzamine C, Indole Alkaloids as cancer cell inhibitors in nutrient starvation, from the Indonesian Marine Sponge Xestospongia muta was done on May 2018. The goal of this study is to determine the cytotoxic activity of metabolite compounds of Xetospongia muta under glucose starvation. The active metabolite isolated using bioassay-guided separation with several steps of chromatography. Interpretation of IR data indicates that the active metabolites have the functional group of secondary NH2 at 3435.56 cm1, CH methyl at 2853.39 cm1, CH methylene at 2769.64 cm1 and CN imine at 1637.27 cm1. The LCMS-ESI analysis showed that the active metabolite has a molecular weight [M+H]+ 347.445 m/z which indicates the active metabolite has a molecular formula C23H29N3. The activity of metabolite compounds against PANC-1 cells showed cytotoxic activity under glucose starvation conditions with values IC50=10 μM, whereas no growth inhibition was observed up to 100 μM under the general culture conditions.