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Sixty seven pellets of Long-eared Owls, Asio otus, were collected in a village, Ehime Prefecture, during 19February 2001to 6 April 2001. The bones of 164 Mus musculus, ten Micromys minutus, two Apodemus speciosus, two Rattus sp., seventeen Pipistrellus abramus, two Myotis macrodactylus, twelve Crocidura dsinezumi and a bird were found in the pellets. M. minutus, P. abramus, M. macrodactylus and C. dsinezumi were new records in diet of the Long-eared owls.