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Donor-derived circulating endothelial cells after kidney transplantation1

Authors :
Eliane R. Popa
Adriana M. Kas-Deelen
Bouke G. Hepkema
Willem J. van Son
T. Hauw The
Martin C. Harmsen
Source :
Transplantation. 74:1320-1327
Publication Year :
2002
Publisher :
Ovid Technologies (Wolters Kluwer Health), 2002.

Abstract

Background. In solid-organ transplantation, the allograft vasculature, in particular the endothelium, is prone to injury inflicted by peritransplantational and posttransplantational factors. Previously, we have shown that circulating endothelial cells (cEC) can be detected in the peripheral blood of kidney allograft recipients and are often associated with acute rejection and active infections with human cytomegalovirus. In the present study we hypothesized that cEC after kidney transplantation are of donor origin, thus reflecting transplantation-related damage to the allograft endothelium. Methods. Using hydraulic micromanipulation equipment, we isolated single cEC (n=153) from the peripheral blood of nine kidney allograft recipients at various time points after transplantation. We demonstrated the origin of these cells (donor or recipient) by typing their HILA-DRB alleles by single-cell, genomic, nested polymerase chain reaction. Results. The majority (71.8%) of cEC were of donor origin and could be detected up to 141 days after onset of acute rejection episodes. Although less frequent (28.2%), recipient-type cEC were detected in the same time course as donor-type cEC. Conclusion. We conclude that posttransplantational injury to the allograft endothelium is reflected by the presence of donor-derived cEC in the blood.

Details

ISSN :
00411337
Volume :
74
Database :
OpenAIRE
Journal :
Transplantation
Accession number :
edsair.doi...........f56b1e1dc0ffd563772bc14ba5aebaae
Full Text :
https://doi.org/10.1097/00007890-200211150-00021