Back to Search
Start Over
Expression of UDP- N -Acetyl-α-<scp>d</scp>-Galactosamine-Polypeptide N -Acetylgalactosaminyltransferase Isozyme 3 in the Subserosal Layer Correlates with Postsurgical Survival of Pathological Tumor Stage 2 Carcinoma of the Gallbladder
- Source :
- Clinical Cancer Research. 10:2090-2099
- Publication Year :
- 2004
- Publisher :
- American Association for Cancer Research (AACR), 2004.
-
Abstract
- Purpose and Experimental Design: Little is known about the molecular events leading to the development and progression of pathological tumor stage 2 (pT2) gallbladder carcinoma. An alteration in the site of O-glycosylation may be associated with malignant behavior of carcinoma cells by modulation of the biological properties of the target mucin. The UDP-N-acetyl-α-d-galactosamine-polypeptide N-acetylgalactosaminyltransferase isozyme 3 (GalNAc-T3) has the epithelial gland-specific expression and catalyzes mucin-type O-glycosylation. In this study, immunohistochemistry was performed to determine the expression level of GalNAc-T3 in 34 cases of pT2 gallbladder carcinoma to determine the correlation of the GalNAc-T3 expression level with mode of recurrence and postsurgical survival. Results: The expression levels of GalNAc-T3 protein and mRNA were increased in gallbladder carcinomas compared with the levels in adjacent noncancerous tissues and in intact gallbladders. Immunostaining of GalNAc-T3 was recognized in the cancerous epithelia, and the subcellular localization was classified into granular and diffuse types. In the 34 cases of pT2 carcinoma, the localization of GalNAc-T3 was granular type in 50% and diffuse type in 50% of the cases at the deepest invading sites in the subserosal layer. Postsurgical recurrence was significantly more frequent in cases showing diffuse-type localization of GalNAc-T3 at the deepest invading sites (65%) than in those showing granular-type localization (23%; P < 0.05). Postsurgical survival was significantly poorer in cases showing diffuse-type localization than in those showing granular-type localization (P = 0.033) Conclusions: In pT2 gallbladder carcinoma, the presence of diffuse-type localization of GalNAc-T3 in the subserosal layer is correlated with aggressiveness of the disease. This phenotype may serve as a unique biological feature associated with the malignant behavior.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Gallbladder
Mucin
Polypeptide N-acetylgalactosaminyltransferase
Biology
medicine.disease
Isozyme
carbohydrates (lipids)
medicine.anatomical_structure
Oncology
Gene expression
Carcinoma
medicine
Immunohistochemistry
lipids (amino acids, peptides, and proteins)
Immunostaining
Subjects
Details
- ISSN :
- 15573265 and 10780432
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Clinical Cancer Research
- Accession number :
- edsair.doi...........f59d4b877a901d102232f9b6132bd2fd