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Ascorbate oxidase ofCucumis meloL. var. reticulatus: purification, characterization and antibody production
- Source :
- Journal of Experimental Botany. 45:717-724
- Publication Year :
- 1994
- Publisher :
- Oxford University Press (OUP), 1994.
-
Abstract
- Ascorbate oxidase activity and ascorbic acid content were followed during the development of muskmelon (Cucumis melo L. var. reticulatus) fruits. The enzyme was highly expressed in ovaries and very young fruit tissues, followed by a decrease in 10- and 20-d-old fruits and an increase in 30- and 35-d-old fruits which coincided with early events of fruit ripening. Ascorbic acid content was negatively correlated with ascorbate oxidase activity. The enzyme was purified to homogeneity following ion exchange, affinity and gel filtration chromatographic trials. The purified enzyme was a glycoprotein of molecular weight 137 000 composed of two subunits of molecular weight 68 000, and formed by six isoenzymes with isoelectric points in the range of pH 7.7 to 8.3
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi...........f62101391dd3c227b3c2f72e206181bd
- Full Text :
- https://doi.org/10.1093/jxb/45.6.717