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Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft

Authors :
Elza Ibrahim Auerkari
Listyowati Listyowati
Mindya Yuniastuti
Lilies Dwi Sulistyani
Yolanda Bilynov
Source :
Pesquisa Brasileira em Odontopediatria e Clínica Integrada. 21
Publication Year :
2021
Publisher :
FapUNIFESP (SciELO), 2021.

Abstract

Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme . The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.

Details

ISSN :
19834632 and 15190501
Volume :
21
Database :
OpenAIRE
Journal :
Pesquisa Brasileira em Odontopediatria e ClĂ­nica Integrada
Accession number :
edsair.doi...........f6b3ea600b600b874dd1e8755bc41f1e
Full Text :
https://doi.org/10.1590/pboci.2021.048