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Strategies for Improving the Protein Yield in pH-Shift Processing of Ulva lactuca Linnaeus: Effects of Ulvan Lyases, pH-Exposure Time, and Temperature
- Source :
- ACS Sustainable Chemistry & Engineering. 7:12688-12691
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Globally, there is a need for novel vegetarian protein sources. We recently showed that the pH-shift process, using alkaline protein solubilization followed by isoelectric precipitation, is an efficient way to produce extracts with high protein concentrations from Ulva lactuca (>50% on a dry matter basis). However, the total protein yield was low, and to improve this, the effects of adding ulvan lyase, preincubating the seaweed homogenate at pH 8.5 and using different protein extraction temperatures (8 °C, RT and 40 °C), were evaluated in this study. Addition of ulvan lyase reduced protein solubility but increased the precipitation. Incubation at pH 8.5, without ulvan lyase added, significantly increased both protein solubility and precipitation at 8 °C and RT. Temperature per se had no effect on protein solubility, while protein precipitation increased with decreasing temperature. Highest protein yield (29%) was achieved when keeping the process at 8 °C with a preincubation step at pH 8.5 for 1 h. By these process modifications, the yield was 3.2 times higher than achieved by the control process (9.2%).
- Subjects :
- biology
Renewable Energy, Sustainability and the Environment
Precipitation (chemistry)
Chemistry
General Chemical Engineering
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
biology.organism_classification
01 natural sciences
0104 chemical sciences
Isoelectric point
Yield (chemistry)
Protein purification
Environmental Chemistry
Protein precipitation
Dry matter
Ulva lactuca
Food science
0210 nano-technology
Incubation
Subjects
Details
- ISSN :
- 21680485
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- ACS Sustainable Chemistry & Engineering
- Accession number :
- edsair.doi...........f79a05f1944e34b8bcca2109a91fb9dc