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Study Genetic Distances Amonge Nine Okra, (Abelmoschus, esculentus) genotypes UsingTen ISSR markers

Authors :
Melath AL- Ghufaili
Taif Razaq Majid
Attyaf Al-Tamimi
Source :
Al-Kufa University Journal for Biology. 12:1-10
Publication Year :
2023
Publisher :
University of Kufa, 2023.

Abstract

Ten from molecular marker ISSRs, (Inter, - Simple, Sequence, Repeats.) were used to find genetic, diversity, genetic* relationship, and DNA* fingerprint* of nine Okra (Abelmoschus esculentus) genotypes. Primers varied among them in giving unique DNA fingerprints, primers (UBC-809, HB12, and HBS10) gave a unique fingerprint for one genotype of okra, while primers (844A, UBC808) give unique fingerprint for four genotypes. High genetic distance was (0.722) between Egypt and Hasnawia while low genetic distance was (0.074) between Hasnawia and Lahluba. Cluster analysis (Phylogenetic tree) grouped studied genotypes in to two main cluster, the first large one included six genotype(Mosulia , Houseagrl, Khnisiraa ,Hasnawia ,Lahluba and Batra) and other small one includes three genotype (Soutl,Egypt and Zasco seed).ISSR marker could reveal genetic relationship in studied genotypes according to their origin, thus it gave an excellent tool to breeding programs help breeders .

Details

ISSN :
23116544 and 20738854
Volume :
12
Database :
OpenAIRE
Journal :
Al-Kufa University Journal for Biology
Accession number :
edsair.doi...........f81f73bdec1408d1a2653824ca2cf384
Full Text :
https://doi.org/10.36320/ajb/v12.i3.11787