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[40] Reduced nicotinamide adenine dinucleotide phosphate: Δ4-3-ketosteroid 5α-oxidoreductase (rat ventral prostate)

[40] Reduced nicotinamide adenine dinucleotide phosphate: Δ4-3-ketosteroid 5α-oxidoreductase (rat ventral prostate)

Authors :
Ronald J. Moore
Jean D. Wilson
Publication Year :
1975
Publisher :
Elsevier, 1975.

Abstract

Publisher Summary The 5α-reduction of radioactive Δ4-3-ketosteroids, which do not contain substitutions on carbon-11 of the steroid molecule, is followed in the presence of an excess of NADPH by measuring the appearance of the radioactive 5α-reduced metabolites, utilizing a rapid thin-layer chromatographic separation. The assay in this chapter has been developed because the low activity of the enzyme in most tissues precludes the measurement of NADPH disappearance spectrophotometrically. The enzyme exhibits distinctive tissue localization in that most activity is found in the organs of accessory reproduction and in liver.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........f8e39bcb2d17b3b219ced444483061f0
Full Text :
https://doi.org/10.1016/s0076-6879(75)36043-6