A nuclear polyhedrosis virus ofHyphantria cunea replicatesin vitro

A nuclear polyhedrosis virus ofHyphantriacunea replicated successfully in theTrichoplusiani cell line. Restriction endonuclease analysis of the viral DNA obtained from infected cell culture showed the same general homogeneity as that from virus isolated from diseased host larvae. Electron microscopic observations showed that the occluded virus from cell culture consists of rod-like nucleocapsids (31×320 nm) enveloped in aggregates and embedded in polyhedral inclusion bodies from 0.6 to 2.5 μm in diameter.