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Anti-Obesity Activity of Saringosterol Isolated fromSargassum muticum(Yendo) Fensholt Extract in 3T3-L1 Cells
- Source :
- Phytotherapy Research. 31:1694-1701
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Saringosterol, a steroid isolated from Sargassum muticum, a brown edible alga widely distributed on the seashores of southern and eastern Korea, has been shown to exhibit anti-obesity effect. In this study, we investigated the anti-obesity activity of saringosterol through various experiments. The inhibitory effect of saringosterol on adipogenesis was evaluated via Oil Red O staining in 3T3-L1 preadipocytes. After confirming that saringosterol is not cytotoxic to these cells by using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, the effect of saringosterol on the expression of various adipogenesis-related genes was analyzed via quantitative real-time polymerase chain reaction and western blotting. We demonstrated that saringosterol dose dependently inhibited adipocyte differentiation and expression of adipogenic marker genes such as adipocyte fatty acid-binding protein, adiponectin, resistin, and fatty acid synthase in 3T3-L1 cells. In addition, saringosterol significantly inhibited the mRNA and protein expression of peroxisome proliferator-activated receptor γ and CCAAT enhancer-binding protein α in 3T3-L1 cells. Collectively, these findings indicate that saringosterol isolated from S. muticum exhibits anti-obesity effect by inhibiting the expression of adipogenic transcription factors and marker genes and that it may be developed as a drug to suppress adipogenesis. Copyright © 2017 John Wiley & Sons, Ltd.
- Subjects :
- 0301 basic medicine
Pharmacology
chemistry.chemical_classification
Adiponectin
biology
Chemistry
Cellular differentiation
Peroxisome proliferator-activated receptor
biology.organism_classification
Molecular biology
Fatty acid-binding protein
03 medical and health sciences
Fatty acid synthase
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Adipogenesis
030220 oncology & carcinogenesis
Adipocyte
biology.protein
Sargassum muticum
Subjects
Details
- ISSN :
- 0951418X
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Phytotherapy Research
- Accession number :
- edsair.doi...........fac15918fcc58bd0d1644de044fc697f