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Osteoinductive potential of recombinant BMP-9 in bone defects of mice treated with antiresorptive agents

Authors :
Fujioka-Kobayashi, M
Marjanowski, S D
Kono, M
Hino, S
Saulacic, N
Schaller, B
Publication Year :
2021
Publisher :
Elsevier, 2021.

Abstract

The aim of the present study was to investigate the effects of recombinant human (rh)BMP-9 on bone regenerative potential in a mouse model of antibody-mediated antiresorptive therapy (AMART). A monoclonal anti-murine receptor activator of nuclear factor-kappa B ligand (RANKL) antibody (mAb) was used to create an AMART model in mice. rhBMP-9 combined with collagen membrane was implanted in calvarial defects in mAb-treated mice. After 4 weeks, the bone formative potential in the defects was evaluated by micro-computed tomography and histological approaches. The groups implanted with rhBMP-9-containing collagen membranes demonstrated substantial osteopromotive potential, with significantly greater new bone volume (Sham + BMP-9 group; 0.86 �� 0.29 mm3 and mAb + BMP-9 group; 0.64 �� 0.16 mm3) than control PBS-membranes (Sham + PBS group; 0.44 �� 0.29 mm3 and mAb + PBS group; 0.24 �� 0.12 mm3) in both sham and mAb-treated mice. In line with in vivo study, bone marrow cells isolated from both sham and mAb-treated mice confirmed greater osteogenic potential upon stimulation with rhBMP-9 in vitro. These findings suggest for the first time that local rhBMP-9 administration might be a strategy to accelerate bone regeneration in the context of AMART.

Subjects

Subjects :
610 Medicine & health

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........fb2b6436c2056b16347bbfb69453afeb
Full Text :
https://doi.org/10.48350/163493