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Dual Protective Effects of Flavonoids from Petasites japonicus against UVB-Induced Apoptosis Mediated via HSF-1 Activated Heat Shock Proteins and Nrf2-Activated Heme Oxygenase-1 Pathways
- Source :
- Biological & Pharmaceutical Bulletin. 40:765-773
- Publication Year :
- 2017
- Publisher :
- Pharmaceutical Society of Japan, 2017.
-
Abstract
- The leaves of Petasites japonicus are used for their anti-allergic properties in traditional Korean, Japanese, and Chinese medicine. This study aimed to identify bioactive compounds isolated from P. japonicus leaves. All compounds were assessed for their ability of transcriptional activation, induction of phase 2 enzymes and heat shock proteins (HSPs), as well as protection against the UVB-induced apoptotic cell death. Bioactive compounds were isolated from P. japonicus leaves. All compounds were evaluated for their protective effect using human dermal fibroblasts (HDF) and human epidermal keratinocyte cells (HEKC) treated with UVB radiation. Four flavonoids were isolated from the leaves of P. japonicus and identified as kaempferol-3-O-(6″-acetyl)-β-D-glucoside (1), quercetin-3-O-(6″-acetyl)-β-D-glucoside (2), kaempferol-3-O-β-D-glucoside (3), and quercetin-3-O-β-D-glucoside (4). These compounds activated nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heat-shock response transcription elements (HSE) that resulted in the induction of heme oxygenase-1 (HO-1) and HSP70, respectively. Activation of these pathways provided protection to the skin cells against UVB radiation. The isolated compounds activated the Nrf2 and HSE pathways and could protect against UVB-induced apoptosis.
- Subjects :
- 0301 basic medicine
Pharmacology
Petasites
integumentary system
Pharmaceutical Science
Petasites japonicus
General Medicine
Biology
biology.organism_classification
Molecular biology
Hsp70
Heme oxygenase
Heat shock factor
03 medical and health sciences
030104 developmental biology
Biochemistry
UVB-induced apoptosis
Apoptosis
Heat shock protein
Subjects
Details
- ISSN :
- 13475215 and 09186158
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Biological & Pharmaceutical Bulletin
- Accession number :
- edsair.doi...........fc626cd927f26fd06b58f62e4598efcd