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Deficiency of immunoglobulin E protects mice from experimental abdominal aortic aneurysms
- Source :
- The FASEB Journal. 34:3091-3104
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Allergic asthma with high plasma IgE levels is a significant risk factor of human abdominal aortic aneurysm (AAA). This study tests a direct role of IgE in angiotensin-II (Ang-II) perfusion- and peri-aortic CaCl2 injury-induced AAA in mice. In both models, IgE-deficiency in Apoe-/- Ige-/- mice blunts AAA growth and reduces lesion accumulation of macrophages, CD4+ and CD8+ T cells, and lesion MHC class-II expression, CD31+ microvessel growth, and media smooth muscle cell loss, compared with those from Apoe-/- control mice. Real time-PCR reveals significant reductions in expression of neutrophil chemoattractants MIP-2α and CXCL5 in AAA lesions or macrophages from Apoe-/- Ige-/- mice, along with reduced lesion Ly6G+ neutrophil accumulation. Consistent with reduced lesion inflammatory cell accumulation, we find significant reductions of plasma and AAA lesion IL6 expression in Apoe-/- Ige-/- mice. Immunofluorescent staining and FACS analysis show that AAA lesion neutrophils express FceR1. Mechanistic study demonstrates that IgE induces neutrophil FceR1 expression, activates MAPK signaling, and promotes IL6 production. This study supports a direct role of IgE in AAA by promoting lesion chemokine expression, inflammatory cell accumulation, MAPK signaling, and cytokine expression. IgE inhibition may represent a novel therapeutic approach in AAA management.
- Subjects :
- 0301 basic medicine
CD31
MAPK/ERK pathway
Chemokine
Pathology
medicine.medical_specialty
Immunoglobulin E
Biochemistry
Lesion
03 medical and health sciences
0302 clinical medicine
Genetics
Medicine
Molecular Biology
biology
business.industry
FCER1
030104 developmental biology
CXCL5
cardiovascular system
biology.protein
medicine.symptom
business
030217 neurology & neurosurgery
CD8
Biotechnology
Subjects
Details
- ISSN :
- 15306860 and 08926638
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The FASEB Journal
- Accession number :
- edsair.doi...........fc9462272cc48fdeb4b5d58d9e31dee3