Model development for microbial spoilage of packaged fresh‒cut salad products using temperature and in-package CO2 levels as predictor variables

The objective of this study was to develop a model system for the prediction of the impact of temperature and in-package % CO2 on the microbial spoilage of fresh‒cut vegetables with high respiration rate independent of packaging characteristics and post-harvest physiology. Model development was based on study of rocket pulp stored under 0–20% O2: 20-0% CO2: 80% N2 at 0–15 °C. Growth of pseudomonads and lactic acid bacteria (LAB) was primary modelled by Baranyi model, while growth rate was further modeled as a function of storage temperature and % CO2 by a polynomial model. Both growth models were validated against various fresh‒cut vegetables of high respiration rate, modified atmosphere packaging, and packaging films, under isothermal and dynamic temperature conditions. Variations of gas concentration of rocket pulp was eliminated or significantly decreased (p