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The activities of some key enzymes of the secondary metabolism in cell suspension cultures of two Humulus lupulus cultivars, namely a Verticillium albo-atrum susceptible (‘Hallertauer Mittelfruh’) and a resistant cultivar (‘Wye Target’), were measured under normal growth conditions and after the addition of a culture filtrate of the fungus V. albo-atrum as elicitor. A marked change was observed upon elicitation in the phenylalanine ammonia lyase activity found for ‘Wye Target’, being almost 2-3 times higher than the control during the first hours after elicitation.