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Prism coupler-based sensor system for simultaneous screening of synthetic glucocorticosteroid as doping control agent
- Source :
- Sensors and Actuators B: Chemical. 260:432-444
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Profiling at GCs level is an important issue since it is a key factor to determine administration intent for either therapeutic or doping purpose. In this study, the sensing platform which is based on a combination of surface plasmon resonance system with molecularly imprinted nanoparticles was developed for monitoring of prednisolone level as a selected GCs analyte in plasma and urine. Fabricated sensing platform was utilized with ellipsometer and contact angle measurements followed by characterization of nanoparticles (NPs) by the means of structurally (i.e. FTIR and elemental analysis) and morphologically (i.e. TEM and Zeta-sizer analysis). The lowest limit of detection by PIN2 encoded molecularly imprinted nanoparticle was achieved as at 5.5 ppb and 8.9 ppb at pH 6.6 and 7.4, respectively. Competition kinetic assays were performed by using 17-α-ethynyl estradiol (EEST) and estradiol (EST). The reusability studies have been applied to the sensor with the best results and 92.8% recovery is achieved. Validation studies of the system were performed according to the standard procedure by high performance liquid chromatography (HPLC). The method was successfully applied to monitor PRD levels in body fluids (urine and plasma) as a natural sample.
- Subjects :
- Detection limit
Analyte
Chromatography
Materials science
010401 analytical chemistry
Metals and Alloys
Nanoparticle
02 engineering and technology
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
High-performance liquid chromatography
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Contact angle
Prism coupler
Elemental analysis
Materials Chemistry
Electrical and Electronic Engineering
Surface plasmon resonance
0210 nano-technology
Instrumentation
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 260
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi...........fe192ab32774bf0bb14a24020681e1f2