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Production of γ-aminobutyric acid from monosodium glutamate using Escherichia coli whole-cell biocatalysis with glutamate decarboxylase from Lactobacillus brevis KCTC 3498

Production of γ-aminobutyric acid from monosodium glutamate using Escherichia coli whole-cell biocatalysis with glutamate decarboxylase from Lactobacillus brevis KCTC 3498

Authors :
Sun Mi Lee
Yung-Hun Yang
Ranjit Gurav
Ye-Lim Park
Hyun-Joong Kim
Hun-Suk Song
Hye Soo Lee
Shashi Kant Bhatia
Jun Young Park
Tae-Rim Choi
Sol Lee Park
Yeong-Hoon Han
Source :
Korean Journal of Chemical Engineering. 37:2225-2231
Publication Year :
2020
Publisher :
Springer Science and Business Media LLC, 2020.

Abstract

γ-Aminobutyric acid (GABA), an important fine chemical in pharmacotherapy and food industries, is used as a novel material in the nylon industry and has attracted attention for its potential application in large scale production. Search for new genes and strains, development of efficient reaction systems, such as fermentation and bioconversion, and use of cheap starting material like monosodium glutamate (MSG) can make GABA production using less expensive bulk chemicals possible. Therefore, in this study, we constructed a recombinant Escherichia coli whole-cell system for GABA production that expressed glutamate decarboxylase (GAD) from Lactobacillus brevis and used MSG as the starting material. We also optimized the reaction conditions for MSG to GABA conversion, such as citrate buffer concentration, pyridoxal 5′te concentration, temperature, MSG concentration, and cell density (OD600). The optimized whole-cell system converted MSG to GABA via seven repetitive cycles resulting in an average conversion rate of 86% (71.7 mM/h) within 42 h.

Details

ISSN :
19757220 and 02561115
Volume :
37
Database :
OpenAIRE
Journal :
Korean Journal of Chemical Engineering
Accession number :
edsair.doi...........fe9ea39272177dd47a74c0841993d1a8
Full Text :
https://doi.org/10.1007/s11814-020-0633-z