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Production of γ-aminobutyric acid from monosodium glutamate using Escherichia coli whole-cell biocatalysis with glutamate decarboxylase from Lactobacillus brevis KCTC 3498
Production of γ-aminobutyric acid from monosodium glutamate using Escherichia coli whole-cell biocatalysis with glutamate decarboxylase from Lactobacillus brevis KCTC 3498
- Source :
- Korean Journal of Chemical Engineering. 37:2225-2231
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- γ-Aminobutyric acid (GABA), an important fine chemical in pharmacotherapy and food industries, is used as a novel material in the nylon industry and has attracted attention for its potential application in large scale production. Search for new genes and strains, development of efficient reaction systems, such as fermentation and bioconversion, and use of cheap starting material like monosodium glutamate (MSG) can make GABA production using less expensive bulk chemicals possible. Therefore, in this study, we constructed a recombinant Escherichia coli whole-cell system for GABA production that expressed glutamate decarboxylase (GAD) from Lactobacillus brevis and used MSG as the starting material. We also optimized the reaction conditions for MSG to GABA conversion, such as citrate buffer concentration, pyridoxal 5′te concentration, temperature, MSG concentration, and cell density (OD600). The optimized whole-cell system converted MSG to GABA via seven repetitive cycles resulting in an average conversion rate of 86% (71.7 mM/h) within 42 h.
- Subjects :
- biology
Bioconversion
Lactobacillus brevis
Monosodium glutamate
General Chemical Engineering
Glutamate decarboxylase
General Chemistry
medicine.disease_cause
biology.organism_classification
Aminobutyric acid
chemistry.chemical_compound
Biochemistry
chemistry
medicine
Fermentation
Escherichia coli
Pyridoxal
Subjects
Details
- ISSN :
- 19757220 and 02561115
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Korean Journal of Chemical Engineering
- Accession number :
- edsair.doi...........fe9ea39272177dd47a74c0841993d1a8
- Full Text :
- https://doi.org/10.1007/s11814-020-0633-z