Efficient protein extraction for proteomics and metaproteomics (also suitable for low biomass samples) v1

This protocol describes an optimized protein extraction method for (meta-) proteomic analyses. It is based on several existing protocols (see references below) that were combined and adapted and is also compatible with low biomass samples. References: Erde et al. 2014, J. Proteome Res. 13, 1885-1895 Kleiner et al. 2017, Nat. Comm. 8, Article Number 1558 Kulak et al. 2014, Nat. Methods 11, 319-324 León et al. 2013, Mol. Cell. Proteomics 12, 2992-3005 Masuda 2008, J. Proteome Res. 7, 731-740 Wang et al. 2015, Int. J. Anal. Chem. 2015,Article ID 763969 Wisniewski et al. 2009, Nat. Methods 6, 359-362