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A New Bioassay Platform Design for the Discovery of Small Molecules with Anticancer Immunotherapeutic Activity

Authors :
Emiliano Manzo
Angelo Fontana
Giusi Barra
Giuliana d'Ippolito
Laura Fioretto
Genoveffa Nuzzo
Carmela Gallo
Raffaele De Palma
Marcello Ziaco
Marisa Saponaro
Gallo, Carmela
Barra, Giusi
Saponaro, Marisa
Manzo, Emiliano
Fioretto, Laura
Ziaco, Marcello
Nuzzo, Genoveffa
D'Ippolito, Giuliana
De Palma, Raffaele
Fontana, Angelo
Source :
Marine Drugs, Vol 18, Iss 604, p 604 (2020), Marine drugs (2020). doi:10.3390/md18120604, info:cnr-pdr/source/autori:Carmela Gallo, Giusi Barra, Marisa Saponaro, Emiliano Manzo, Laura Fioretto, Marcello Ziaco, Genoveffa Nuzzo, Giuliana d'Ippolito, Raffaele De Palma, Angelo Fontana/titolo:A New Bioassay Platform Design for the Discovery of Small Molecules with Anticancer Immunotherapeutic Activity/doi:10.3390%2Fmd18120604/rivista:Marine drugs/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume, Marine Drugs, Volume 18, Issue 12
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for the preclinical discovery of anticancer candidates. The platform relies on the use of dendritic cells that are professional antigen-presenting cells (APC) able to activate T cells and trigger a primary adaptive immune response. The experimental procedure is based on two phenotypic assays for the selection of chemical leads by both a panel of nine tumor cell lines and growth factor-dependent immature mouse dendritic cells (D1). The positive hits are then validated by a secondary test on human monocyte-derived dendritic cells (MoDCs). The aim of this approach is the selection of potential immunotherapeutic small molecules from natural extracts or chemical libraries.

Details

Language :
English
ISSN :
16603397
Volume :
18
Issue :
604
Database :
OpenAIRE
Journal :
Marine Drugs
Accession number :
edsair.doi.dedup.....003d66a8d84d7c6919c05353c9881a2a
Full Text :
https://doi.org/10.3390/md18120604