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New candidate tumor-suppressor gene KLF6 and its splice variant KLF6 SV2 counterbalancing expression in primary hepatocarcinoma
- Source :
- Hepato-gastroenterology. 59(114)
- Publication Year :
- 2011
-
Abstract
- BACKGROUND/AIMS This study aimed to detect the expression of newly discovered zinc finger transcriptional factor KLF6 and its splice variant KLF6 SV2 in primary hepatocarcinoma (PHC) tissues and hepatoma cell strains, and to evaluate their clinicopathologic relationship with PHC. METHODOLOGY Wild-type KLF6 and KLF6 SV2 mRNA expression was determined by RTPCR in 27 cases of PHC tissues and cell strains of HepG2, SMMC7721 and LO2. Western blotting and immunohistochemical staining were adopted to detect KLF6 protein expression. Positive area ratio of wild-type KLF6 protein expression and its relationship with clinicopathological parameters of PHC was analyzed. RESULTS Wild-type KLF6 expression in PHC tissues was lower than that in paracancerous tissues. In contrast, KLF6 SV2 mRNA expression was higher in PHC tissues and hepatoma cell strains (p
- Subjects :
- Liver Cirrhosis
Male
Carcinoma, Hepatocellular
Cellular differentiation
education
Cell
Blotting, Western
Kruppel-Like Transcription Factors
behavioral disciplines and activities
Metastasis
Proto-Oncogene Proteins
medicine
Kruppel-Like Factor 6
Gene silencing
Humans
Protein Isoforms
Gene Silencing
RNA, Messenger
Regulation of gene expression
Venous Thrombosis
Hepatology
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Proteins
Liver Neoplasms
Gastroenterology
Cell Differentiation
General Medicine
Hep G2 Cells
Middle Aged
medicine.disease
Immunohistochemistry
Tumor Burden
Gene Expression Regulation, Neoplastic
Real-time polymerase chain reaction
medicine.anatomical_structure
KLF6
Lymphatic Metastasis
Cancer research
Female
business
Subjects
Details
- ISSN :
- 01726390
- Volume :
- 59
- Issue :
- 114
- Database :
- OpenAIRE
- Journal :
- Hepato-gastroenterology
- Accession number :
- edsair.doi.dedup.....003e10ed8ab838bddb46e6e07839a369