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Critical function of DNA methyltransferase 1 in tomato development and regulation of the DNA methylome and transcriptome
- Source :
- Journal of integrative plant biologyREFERENCES. 61(12)
- Publication Year :
- 2019
-
Abstract
- DNA methylation confers epigenetic regulation on gene expression and thereby on various biological processes. Tomato has emerged as an excellent system to study the function of DNA methylation in plant development. To date, regulation and function of DNA methylation maintenance remains unclear in tomato plants. Here, we report the critical function of tomato (Solanum lycopersicum) Methyltransferase 1 (SlMET1) in plant development and DNA methylome and transcriptome regulation. Using CRISPR-Cas9 gene editing, we generated slmet1 mutants and observed severe developmental defects with a frame-shift mutation, including small and curly leaves, defective inflorescence, and parthenocarpy. In leaf tissues, mutations in SlMET1 caused CG hypomethylation and CHH hypermethylation on a whole-genome scale, leading to a disturbed transcriptome including ectopic expression of many RIN target genes such as ACC2 in leaf tissues, which are normally expressed in fruits. Neither the CG hypomethylation nor CHH hypermethylation in the slmet1 mutants is related to tissue culture. Meanwhile, tissue culture induces non-CG hypomethylation, which occurs more frequently at gene regions than at TE regions. Our results depict SlMET1- and tissue culture-dependent tomato DNA methylomes, and that SlMET1 is required for maintaining a normal transcriptome and normal development of tomato.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Transcription, Genetic
Plant Science
Biology
Environment
medicine.disease_cause
01 natural sciences
Biochemistry
DNA methyltransferase
General Biochemistry, Genetics and Molecular Biology
Transcriptome
03 medical and health sciences
Solanum lycopersicum
Gene Expression Regulation, Plant
medicine
Epigenetics
Gene
Plant Proteins
Regulation of gene expression
Gene Editing
Mutation
Base Sequence
fungi
food and beverages
Gene Expression Regulation, Developmental
Genetic Pleiotropy
Methyltransferases
DNA Methylation
Cell biology
030104 developmental biology
Phenotype
DNA methylation
Ectopic expression
CpG Islands
Genome, Plant
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17447909
- Volume :
- 61
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of integrative plant biologyREFERENCES
- Accession number :
- edsair.doi.dedup.....004fec1ef06702a23bdba9a0722f86fe