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Diacylglycerol kinase η colocalizes and interacts with apoptosis signal-regulating kinase 3 in response to osmotic shock
- Source :
- Biochemistry and Biophysics Reports, Vol 26, Iss, Pp 101006-(2021)
- Publication Year :
- 2021
-
Abstract
- Diacylglycerol kinase (DGK) η translocates from the cytoplasm to punctate vehicles via osmotic shock. Apoptosis signal-regulating kinase (ASK) 3 (MAP kinase kinase kinase (MAPKKK) 15) is also reported to respond to osmotic shock. Therefore, in the present study, we examined the subcellular localization of DGKη and ASK3 expressed in COS-7 cells under osmotic stress. We found that DGKη was almost completely colocalized with ASK3 in punctate structures in response to osmotic shock. In contrast, DGKδ, which is closely related to DGKη structurally, was not colocalized with ASK3, and DGKη failed to colocalize with another MAPKKK, C-Raf, even under osmotic stress. The structures in which DGKη and ASK3 localized were not stained with stress granule makers. Notably, DGKη strongly interacted with ASK3 in an osmotic shock-dependent manner. These results indicate that DGKη and ASK3 undergo osmotic shock-dependent colocalization and associate with each other in specialized structures.
- Subjects :
- 0301 basic medicine
Osmotic shock
MAP kinase kinase kinase
Kinase
Chemistry
QH301-705.5
Diacylglycerol kinase
Biophysics
Colocalization
QD415-436
Subcellular localization
Biochemistry
Cell biology
C-Raf
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Stress granule
030220 oncology & carcinogenesis
c-Raf
Biology (General)
Apoptosis signal-regulating kinase
Subjects
Details
- ISSN :
- 24055808
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Biochemistry and biophysics reports
- Accession number :
- edsair.doi.dedup.....0063c7564ac6edb022b8c200a01860b8