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Phase image correlation spectroscopy for detecting microfluidic dynamics

Authors :
Lan Yu
Yu Wang
Yang Wang
Kequn Zhuo
Ying Ma
Min Liu
Juanjuan Zheng
Jianlang Li
Junhua Li
Peng Gao
Source :
Applied Optics. 61:5944
Publication Year :
2022
Publisher :
Optica Publishing Group, 2022.

Abstract

It is essential to quantify the physical properties and the dynamics of flowing particles in many fields, especially in microfluidic-related applications. We propose phase image correlation spectroscopy (PICS) as a versatile tool to quantify the concentration, hydro-diameter, and flow velocity of unlabeled particles by correlating the pixels of the phase images taken on flowing particles in a microfluidic device. Compared with conventional image correlation spectroscopy, PICS is minimally invasive, relatively simple, and more efficient, since it utilizes the intrinsic phase of the particles to provide a contrast instead of fluorescent labeling. We demonstrate the feasibility of PICS by measuring flowing polymethylmethacrylate (PMMA) microspheres and yeast in a microfluidic device. We can envisage that PICS will become an essential inspection tool in biomedicine and industry.

Details

ISSN :
21553165 and 1559128X
Volume :
61
Database :
OpenAIRE
Journal :
Applied Optics
Accession number :
edsair.doi.dedup.....008d94c5091348dddec93b739aceb608