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Elevated Levels of miR-155 in Blood and Urine from Patients with Nephrolithiasis
- Source :
- BioMed Research International, BioMed Research International, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Hindawi Limited, 2014.
-
Abstract
- Background.Both circulating and urinary miRNAs may represent a potential noninvasive molecular biomarker capable of predicting chronic kidney disease, and, in the present study, we will investigate the serum and urinary levels of miR-155 in patients with nephrolithiasis.Methods.Serum and urinary levels of miR-155 are quantified in 60 patients with nephrolithiasis; the result was compared to 50 healthy volunteers. Estimated glomerular filtration (eGFR) was calculated and, by simple regression analysis, the correlations of miR-155/eGFR and miR-155/CRP (C-reactive protein) levels were analyzed as well.Results.The median levels of serum and urinary levels of miR-155 are significantly higher in nephrolithiasis patients than in controls. eGFR inversely correlates with urinary level of miR-155; CRP positively correlates with urinary miR-155. Urinary level of miR-155 inversely correlates with urinary expression of interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-)αand positively correlates with urinary expression of regulated upon activation, normal T-cell expressed, and secreted (RANTES).Conclusion.Serum and urinary levels of miR-155 were significantly elevated in patients with nephrolithiasis, and the upregulation of miR-155 was correlated with decline of eGFR and elevation of CRP. Our results suggested that miR-155 might play important roles in the pathophysiology of nephrolithiasis via regulating inflammatory cytokines expression. Further study on the molecular pathogenic mechanism and larger scale of clinical trial are required.
- Subjects :
- Male
medicine.medical_specialty
Article Subject
Urinary system
lcsh:Medicine
Renal function
Urine
Nephrolithiasis
General Biochemistry, Genetics and Molecular Biology
Proinflammatory cytokine
Internal medicine
medicine
Humans
General Immunology and Microbiology
biology
lcsh:R
C-reactive protein
Case-control study
Interleukin
General Medicine
Middle Aged
medicine.disease
MicroRNAs
C-Reactive Protein
Endocrinology
Gene Expression Regulation
Case-Control Studies
biology.protein
Cytokines
Female
Glomerular Filtration Rate
Research Article
Kidney disease
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....00ca3041d1ac2e50083daae5d7423b3b