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Immunohistochemical examination of proliferative potentials and the expression of cell cycle-related proteins of intracranial chordomas
- Source :
- Human Pathology. 28:714-719
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- The difference in biological features between recurrent and nonrecurrent intracranial chordomas has not been studied. In this study, proliferative potentials of chordomas were studied with an immunohistochemical staining method, mainly using anti-Ki-67 antibody, MIB-1, which is known to be available for archival paraffin sections, together with immunohistochemical studies on the expression of cell cycle or apoptosis-related proteins, including p53, cyclin D1, and bcl-2 proteins. The correlation among MIB-1 staining indices, the immunoreactivities of these proteins, and clinical courses of intracranial chordomas were analyzed retrospectively, and the statistically significant correlation between MIB-1 staining index (SI) and recurrence has been clarified. The mean MIB-1 SI of recurrent tumors was 10.2%, being shown to be higher than that of nonrecurrent tumors (2.8%). The immunohistochemically positive staining of cell cycle-related protein, especially p53 and cyclin D1 proteins, correlated well with recurrence and high MIB-1 SI. In conclusion, both the examination of proliferative potentials of chordomas using MIB-1 SI and the study of the immunoreactivity of p53 and cyclin D1 proteins are important for their biological and histopathological analyses and the prediction of future recurrence.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Adolescent
Tumor suppressor gene
Cell
Biology
Pathology and Forensic Medicine
Histones
Sex Factors
Cyclin D1
Cyclins
Chordoma
medicine
Humans
neoplasms
In Situ Hybridization
Retrospective Studies
Cyclin
Oncogene Proteins
Brain Neoplasms
Nuclear Proteins
Antigens, Nuclear
Middle Aged
Cell cycle
medicine.disease
Immunohistochemistry
digestive system diseases
Staining
Ki-67 Antigen
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
Cancer research
Female
Neoplasm Recurrence, Local
Tumor Suppressor Protein p53
Biomarkers
Cell Division
Subjects
Details
- ISSN :
- 00468177
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Human Pathology
- Accession number :
- edsair.doi.dedup.....00d088852fc3e44f1972b6d9839dedcb