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Preparation of new molecularly imprinted quartz crystal microbalance hybride sensor system for 8-hydroxy-2′-deoxyguanosine determination
- Source :
- Analytica Chimica Acta. 640:82-86
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- WOS: 000265763500012<br />PubMed ID: 19362624<br />The routine measurement of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in biological samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. A new 8-OHdG imprinted quartz crystal microbalance (QCM) sensor has been developed for selective determination of 8-OHdG in serum samples. To fulfil the desired results, we have used methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] and methacryloyl histidine-Pt(II) [MAH-Pt(II)] as metal-chelating monomers via double metal coordination-chelation interactions for the preparation of additional selective molecular imprinted polymers (MIP). The study includes the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM chip. The obtained results have showed that the application of double metal-chelate monomer systems has been more effective than single metal-chelate monomer systems. In this study, the detection limit and the linear working range were found to be 0.0075 and 0.0100-3.5 mu M, respectively. The affinity constant (K-affinity) was found to be 1.54 x 10(5) M-1 for 8-OHdG using MAH-Pt-8-OHdG-MAAP-Fe based thin film. Also, selectivity of prepared QCM sensor was found as being very high in the presence of competitive species. At the last step of this procedure, 8-OHdG level in blood serum which belongs to a intestinal cancer patient was determined by the prepared QCM sensor
- Subjects :
- Analyte
Quartz Crystal Microbalance
Surface Properties
Molecular Imprinted Polymers
Ligands
Sensitivity and Specificity
Biochemistry
Chemistry Techniques, Analytical
Analytical Chemistry
Molecular Imprinting
chemistry.chemical_compound
Blood serum
Humans
Environmental Chemistry
Electrodes
Spectroscopy
Chelating Agents
Detection limit
Chromatography
Metal-Chelate Interaction
Deoxyguanosine
Quartz
Quartz crystal microbalance
Working range
Monomer
chemistry
8-Hydroxy-2 '-Deoxyguanosine
8-Hydroxy-2'-Deoxyguanosine
Metals
Selectivity
Molecular imprinting
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 640
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....00f8549dad1f90bc3781d215b0688190