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Tao‐Hong‐Si‐Wu decoction reduces ischemia reperfusion rat myoblast cells calcium overloading and inflammation through the Wnt/IP3R/CAMKII pathway
- Source :
- Journal of Cellular Biochemistry. 120:13095-13106
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Limb ischemia reperfusion (LIRI) injury is associated with serious local and systemic effects. Reperfusion may augment tissue injury in excess of that produced by ischemia alone. Calcium overloading and inflammation are considered to be two of the pathological mechanisms of limb ischemia/reperfusion (I/R) injury. Tao-Hong-Si-Wu decoction (THSWD) is a traditional Chinese herbal medicine with a powerful anti-inflammatory properties. We studied the probable restorative effect of THSWD on limb I/R-induced calcium overloading and inflammation in myoblast obtained from gastrocnemius muscle tissues of Sprague-Dawley rats (Frizzled Z5,a wnt5a blocker; KN-93, a calmodulin-dependent protein kinase II (CamkII) blocker; XeC, a IP3R blocker as positive controls). The simulated ischemia and reperfusion(I/R) solutions were used to imitate LIRI environment. The results showed that after I/R treatment, the secretion of proinflammatory factors (TNF-α and IL-1β) and Wnt5a/Ca2+ signal molecules (wnt5a, camkII, and IP3R) upregulated significantly, the Ca2+ concentration enhanced too in myoblast cells. THSWD pretreatment decreased the secretion of TNF-α and IL-1β, Ca2+ concentration; and abated the Wnt5a/Ca2+ signal molecules of wnt5a, camkII and IP3R expression activated by I/R injury; but could not abated the Wnt11 and protein kinase C (PKC) expression significantly, the results was similar with Frizzled Z5 treatment cells. Our research illustrated that THSWD may have a mitigating effect on LIRI targeting Wnt/IP3R/CAMKII but not Wnt/IP3R/PKC signaling pathway for the first time. This study may encourage the use of THSWD in the critical clinical settings with LIRI.
- Subjects :
- Male
0301 basic medicine
Frizzled
Ischemia
Gene Expression
chemistry.chemical_element
Inflammation
Calcium
Pharmacology
Biochemistry
Wnt-5a Protein
Proinflammatory cytokine
Myoblasts
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Ca2+/calmodulin-dependent protein kinase
medicine
Animals
Inositol 1,4,5-Trisphosphate Receptors
Molecular Biology
Protein kinase C
Wnt signaling pathway
Proteins
Cell Biology
medicine.disease
030104 developmental biology
chemistry
Reperfusion Injury
030220 oncology & carcinogenesis
medicine.symptom
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Drugs, Chinese Herbal
Phytotherapy
Signal Transduction
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....00fead1de5802dea68fa36ac5db10e7c