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Target MicroRNA-Responsive DNA Hydrogel-Based Surface-Enhanced Raman Scattering Sensor Arrays for MicroRNA-Marked Cancer Screening
- Source :
- Analytical Chemistry. 92:2649-2655
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- On the basis of a target microRNA (miRNA)-responsive DNA hydrogel, a novel surface-enhanced Raman scattering (SERS) sensor array with nine sensor units that can detect multiple cancer-related miRNAs in one sample was developed. The target miRNA-responsive DNA hydrogel was first formed in each sensor unit to realize the construction of the DNA hydrogel-based SERS sensor array. Initially, because of the blocking of the streptavidin (SA)-modified sensor units by the formed DNA hydrogel, the SERS tags (biotin/4-mercaptobenzonitrile-functionalized AuAg alloy nanoparticles (B/M-AuAgNPs)) could not pass through the hydrogel and bind to the SA-modified sensor surface; thus, obvious Raman signals could not be observed. After the introduction of the target miRNA, DNA hydrogels of the corresponding sensor unit were disintegrated accordingly, and SERS tags were able to pass through the hydrogel to be captured onto the SA-modified detection surface, thus resulting in strong Raman signals and the detection of target miRNA. The assay is validated under clean buffer conditions as well as in serum. This target miRNA-responsive DNA hydrogel-based SERS sensor array has attractive application prospects in cancer typing via blood miRNA measurements.
- Subjects :
- Streptavidin
Surface Properties
Nanoparticle
Biosensing Techniques
macromolecular substances
Spectrum Analysis, Raman
010402 general chemistry
complex mixtures
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
symbols.namesake
Sensor array
Biotin
Neoplasms
Biomarkers, Tumor
Humans
Chemistry
010401 analytical chemistry
technology, industry, and agriculture
Hydrogels
DNA
0104 chemical sciences
MicroRNAs
Self-healing hydrogels
Biophysics
symbols
Raman spectroscopy
Raman scattering
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....01134d47602868ce4032da262125a0c8