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Enzymatic production of oroxylin A and hispidulin using a liverwort flavone 6-O-methyltransferase
- Source :
- FEBS letters. 590(16)
- Publication Year :
- 2016
-
Abstract
- Oroxylin A and hispidulin, compounds which are abundant in both Scutellaria and liverwort species, are important lead compounds for the treatment of ischemic cerebrovascular disease. Their enzymatic synthesis requires an O-methyltransferase able to interact with the related flavonoid's 6-OH group, but such an enzyme has yet to be identified in plants. Here, the gene encoding an O-methyltransferase (designated PaF6OMT) was isolated from the liverwort species Plagiochasma appendiculatum. A test of alternative substrates revealed that its strongest preferences were baicalein and scutellarein, which were converted into, respectively, oroxylin A and hispidulin. Allowed a sufficient reaction time, the conversion rate of these two substrates was, respectively, 90% and 100%. PaF6OMT offers an enzymatic route to the synthesis of oroxylin A and hispidulin.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Hepatophyta
Flavonoid
Biophysics
Bioinformatics
01 natural sciences
Biochemistry
Substrate Specificity
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
Genetics
Humans
Apigenin
Cloning, Molecular
Molecular Biology
chemistry.chemical_classification
Flavonoids
biology
Chemistry
Scutellarein
Cell Biology
Methyltransferases
biology.organism_classification
Flavones
O-methyltransferase
Baicalein
Cerebrovascular Disorders
030104 developmental biology
Enzyme
Flavanones
biology.protein
Scutellaria
Oroxylin A
Hispidulin
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18733468
- Volume :
- 590
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....011cd6db5559010f9ad7d8188b2dadf8