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3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices

3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices

Authors :
Arto Heiskanen
Roger A. Barker
Malin Parmar
Alberto Martínez-Serrano
Sebastian Buchmann
Jenny Emnéus
Janko Kajtez
Shashank Vasudevan
Marcella Birtele
Johan Ulrik Lind
Christian Jonathan Pless
Stefano Rocchetti
European Commission
Lund University
Kajtez, Janko [0000-0001-9997-2325]
Vasudevan, Shashank [0000-0001-6490-3434]
Apollo - University of Cambridge Repository
Source :
Kajtez, J, Buchmann, S, Vasudevan, S, Birtele, M, Rocchetti, S, Pless, C J, Heiskanen, A, Barker, R A, Martínez-Serrano, A, Parmar, M, Lind, J U & Emnéus, J 2020, ' 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices ', Advanced Science, vol. 7, no. 16, 2001150 . https://doi.org/10.1002/advs.202001150, Advanced Science, Advanced Science, Vol 7, Iss 16, Pp n/a-n/a (2020), Advanced Science, Vol 8, Iss 12, Pp n/a-n/a (2021), Digital.CSIC. Repositorio Institucional del CSIC, instname
Publication Year :
2020

Abstract

Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by the lack of a simple fabrication approach for the creation of intricate device architectures with high‐aspect ratio features. Here, a hybrid additive manufacturing approach is presented for the fabrication of open‐well compartmentalized neural devices that provides larger freedom of device design, removes the need for manual postprocessing, and allows an increase in the biocompatibility of the system. Suitability of the method for multimaterial integration allows to tailor the device architecture for the long‐term maintenance of healthy human stem‐cell derived neurons and astrocytes, spanning at least 40 days. Leveraging fast‐prototyping capabilities at both micro and macroscale, a proof‐of‐principle human in vitro model of the nigrostriatal pathway is created. By presenting a route for novel materials and unique architectures in microfluidic systems, the method provides new possibilities in biological research beyond neuroscience applications.<br />In this study, a hybrid additive manufacturing approach to soft lithography is developed for the fabrication of open‐well compartmentalized microfluidic devices used to engineer human stem‐cell derived neural networks in vitro. The approach provides larger freedom of design, removes the need for manual postprocessing, increases the biocompatibility of the system, and enables fast prototyping at the micro and macroscale.

Details

Language :
English
Database :
OpenAIRE
Journal :
Kajtez, J, Buchmann, S, Vasudevan, S, Birtele, M, Rocchetti, S, Pless, C J, Heiskanen, A, Barker, R A, Martínez-Serrano, A, Parmar, M, Lind, J U & Emnéus, J 2020, ' 3D-Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices ', Advanced Science, vol. 7, no. 16, 2001150 . https://doi.org/10.1002/advs.202001150, Advanced Science, Advanced Science, Vol 7, Iss 16, Pp n/a-n/a (2020), Advanced Science, Vol 8, Iss 12, Pp n/a-n/a (2021), Digital.CSIC. Repositorio Institucional del CSIC, instname
Accession number :
edsair.doi.dedup.....011d4e5cc95c89ffa23714cde1d0153e
Full Text :
https://doi.org/10.1002/advs.202001150