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Infrared based saliva screening test for COVID‐19
- Source :
- Angewandte Chemie (International Ed. in English), Articles
- Publication Year :
- 2021
- Publisher :
- John Wiley and Sons Inc., 2021.
-
Abstract
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.
- Subjects :
- Saliva
Materials science
Coronavirus disease 2019 (COVID-19)
Spectrophotometry, Infrared
Infrared
Point-of-care testing
Infrared spectroscopy
010402 general chemistry
01 natural sciences
Proof of Concept Study
Sensitivity and Specificity
Catalysis
Specimen Handling
Cohort Studies
03 medical and health sciences
0302 clinical medicine
COVID-19 Testing
Spectrophotometry
Chlorocebus aethiops
medicine
COVID-19 diagnostic, infrared spectroscopy, Raman spectroscopy, saliva, SARS-CoV-2 virions
Animals
Humans
QD
Least-Squares Analysis
Vero Cells
Mass screening
030304 developmental biology
Detection limit
0303 health sciences
saliva
Chromatography
medicine.diagnostic_test
010405 organic chemistry
SARS-CoV-2
COVID-19
Discriminant Analysis
General Chemistry
030206 dentistry
General Medicine
0104 chemical sciences
3. Good health
Point-of-Care Testing
Monte Carlo Method
ATR-FTIR
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15213773 and 14337851
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie (International Ed. in English)
- Accession number :
- edsair.doi.dedup.....011dc883f9c16eafa84da6305b4f5af1