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Complete genome sequence of Mycobacterium Mya-zh01, an endophytic bacterium, promotes plant growth and seed germination isolated from flower stalk of Doritaenopsis
- Source :
- Archives of microbiology. 202(7)
- Publication Year :
- 2019
-
Abstract
- No genomic sequence of Mycobacterium isolated from orchids has been reported yet; therefore, this study intends to analyze the complete genomic sequence of a growth-promoting Mycobacterium from orchid Doritaenopsis. Mycobacterium strain Mya-zh01 was isolated from the flower stalk of Doritaenopsis Jiuhbao Red Rose. Our results show that Mya-zh01 can effectively produce and secrete the plant growth hormone indole-3-acetic acid (IAA). Inoculation of Mya-zh01 increased root number and length, plant height, leaf number, and leaf length in Doritaenopsis. Furthermore, inoculation of Mya-zh01 promotes seed germination in Doritaenopsis. We sequenced and assembled chromosome for Mya-zh01 (5,027,704 bp with 68.48% GC content), which was predicted to encode 4968 proteins with functions in oxidation reduction, growth, plasma membrane, ATP and DNA binding, carbon metabolism and biosynthesis of amino acids pathways. Mya-zh01 may trap iron from nature or host cells to facilitate the growth of the orchids by producing two siderophores (Mycobactin and Nocobactin NA). Four pathways (tryptamine, indole-3-acetamide, indole-3-pyruvate, and flavin monooxygenase) and seven enzymes [tryptophan synthase alpha chain, tryptophan synthase beta chain, amidase, monoamine oxidase, indole-3-pyruvate monooxygenase, indole-3-pyruvate decarboxylase and aldehyde dehydrogenase (NAD +)] involved in IAA biosynthesis were predicted in Mya-zh01 genome. In conclusion, this study demonstrated the significance of Mya-zh01 in facilitating plant growth and seed germination in Doritaenopsis by IAA biosynthesis, which provides a new insight into the mechanism of plant–bacteria interaction in Doritaenopsis.
- Subjects :
- Siderophore
Doritaenopsis
Plant Development
Mycobactin
Tryptophan synthase
Germination
Flowers
Biochemistry
Microbiology
Amidase
Mycobacterium
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Genetics
Orchidaceae
Molecular Biology
030304 developmental biology
0303 health sciences
biology
030306 microbiology
food and beverages
General Medicine
Monooxygenase
biology.organism_classification
chemistry
Seeds
biology.protein
Subjects
Details
- ISSN :
- 1432072X
- Volume :
- 202
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Archives of microbiology
- Accession number :
- edsair.doi.dedup.....01237933616ae227fd1cae2fbedfced4